This document specifies a method for sampling and handling earthworms from field soils as a prerequisite for using these animals as bioindicators (e.g. to assess the quality of a soil as a habitat for organisms). This document applies to all terrestrial biotopes in which earthworms occur. The sampling design of field studies in general is given in ISO 18400-101 and guidance on the determination of effects of pollutants on earthworms in field situations is given in ISO 11268-3. These aspects can vary according to the national requirements or the climatic/regional conditions of the site to be sampled (see also Annex C). This document is not applicable for semi-terrestrial soils and it can be difficult to use under extreme climatic or geographical conditions (e.g. in high mountains). Methods for some other soil organism groups, such as collembolans, are covered in other parts of ISO 23611.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Procedure
7.1 Sampling of the earthworms
7.2 Preservation
7.3 Determination of biomass
8 Data assessment
9 Test report
Annex A Other methods for sampling (informative)
Annex B Species determination in earthworms (informative)
Annex C The modified TSBF method (informative)
Annex D Determination of maximum water-holding capacity (normative)
Annex E Examples of earthworm monitoring programmes (including presentation of their results) (informative)
ISO 10390:2005 Soil quality -- Determination of pH
ISO 10694:1995 Soil quality -- Determination of organic and total carbon after dry combustion (elementary analysis)
ISO 11260:1994 Soil quality -- Determination of effective cation exchange capacity and base saturation level using barium chloride solution
ISO 10390:2005 Soil quality -- Determination of pH
ISO 10694:1995 Soil quality -- Determination of organic and total carbon after dry combustion (elementary analysis)
ISO 11260:1994 Soil quality -- Determination of effective cation exchange capacity and base saturation level using barium chloride solution
ISO 11277:2009 Soil quality -- Determination of particle size distribution in mineral soil material -- Method by sieving and sedimentation
ISO 11465:1993 Soil quality -- Determination of dry matter and water content on a mass basis -- Gravimetric method