This document specifies quantitative test methods to determine the antibacterial activity of all antibacterial textile products including nonwovens. This document is applicable to all textile products, including cloth, wadding, thread and material for clothing, bedclothes, home furnishings and miscellaneous goods, regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting). This document covers three inoculation methods for the determination of antibacterial activity: a) absorption method (an evaluation method in which the test bacterial suspension is inoculated directly onto specimens); b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto specimens); c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto specimens). NOTE Based on the intended application and on the environment in which the textile product is to be used, and also on the surface properties of the textile properties, the user can select the most suitable inoculation method. This document also specifies the colony plate count method and the adenosine triphosphate (ATP) luminescence method for measuring the enumeration of bacteria.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Safety precaution
5 Apparatus
6 Reagents and culture media
6.1 Water
6.2 Tryptone soya broth (TSB)
6.3 Tryptone soya agar (TSA)
6.4 Agar for transfer
6.5 Nutrient broth (NB)
6.6 Peptone salt solution
6.7 Physiological saline
6.8 Soybean-Casein Digest Broth with Lecithin & Polysorbate 80 (SCDLP) medium
6.9 Dilution buffer for shake-out bacterial suspension
6.10 Neutralizing solution
6.11 Enumeration agar (EA)
6.12 Agar for printing
6.13 Cryoprotective solution for bacterial species
6.14 Stock solution of ATP standard reagent
6.15 Buffer solution for ATP luminescent reagent
6.16 ATP luminescent reagent
6.17 ATP extracting reagent
6.18 ATP eliminating reagent
6.19 SCDLP or other medium for preparing ATP reference solution
6.20 Shake-out physiological saline
7 Reference strains
7.1 Strains
7.2 Restoration and storage of strains
8 Test procedures
8.1 Absorption method (see Annex E)
8.2 Transfer method (see Annex E)
8.3 Printing method (see Annex E)
9 Judgement of antibacterial efficacy
10 Test report
Annex A Strain numbers (normative)
Annex B Shaking method (normative)
Annex C Quantitative measurement by plate count method (normative)
Annex D Quantitative measurement by luminescence method (normative)
Annex E Testing examples (informative)
Annex F Efficacy of antibacterial activity (informative)