Water quality. Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test). Part 1: Method using freshly prepared bacteria (ISO 11348-1:2007)
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ISO 11348 describes three methods for determining the inhibition of the luminescence emitted by the marine bacterium Vibrio fischeri (NRRL B-11177). ISO 11348-1:2007 specifies a method using freshly prepared bacteria. This method is applicable to:
waste water; aqueous extracts and leachates; fresh water (surface and ground water); sea and brackish water; eluates of sediment (fresh water, brackish and sea water); pore water; single substances, diluted in water.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Principle
4 Interferences
5 Reagents and materials
6 Apparatus
7 Sampling and sample pretreatment
7.1 Sampling
7.2 Sample preparation
8 Cultivation of luminescent bacteria
8.1 Stock culturing
8.2 Preparation of pre-cultures
8.3 Preparation of main culture
8.4 Preparation of stock suspension
9 Procedure
10 Evaluation
10.1 Inhibitory effect on luminescent bacteria
10.2 Determination of EC-values
11 Expression of results
12 Criteria of validity
13 Precision
14 Test report
Annex A Colour-correction method (informative)
Annex B Dilution level D – Preparation of the dilution series (informative)
Annex C Precision data (informative)
Annex D Testing salt water samples with the luminescent bacteria test with freshly cultured bacteria (informative)
ISO 5667-16:1998 Water quality -- Sampling -- Part 16: Guidance on biotesting of samples
ISO 5814:1990 Water quality -- Determination of dissolved oxygen -- Electrochemical probe method
ISO 7027-1:2016 Water quality -- Determination of turbidity -- Part 1: Quantitative methods
ISO 5667-16:1998 Water quality -- Sampling -- Part 16: Guidance on biotesting of samples
ISO 5814:1990 Water quality -- Determination of dissolved oxygen -- Electrochemical probe method
ISO 7027-1:2016 Water quality -- Determination of turbidity -- Part 1: Quantitative methods