ISO 15088:2007 specifies a method for the determination of degrees of dilution or concentrations as a measure of the acute toxic effect of waste water to fish eggs within 48 h. It is also applicable to treated municipal waste water and industrial effluents.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Interferences
5 Principle
6 Reagents
6.5 Dilution water
7 Apparatus
8 Procedure
8.1 Keeping of fish and production of eggs
8.2 Providing eggs for the test
8.3 Test procedure
9 Determination of toxicological endpoints
9.1 General
9.2 Number of coagulated eggs
9.3 Tail detachment
9.4 Heartbeat
10 Validity criteria
11 Determination of LIDegg
12 Expression of results
13 Test report
14 Reproducibility
Annex A Scheme for layout and distribution of eggs in microplates (informative)
ISO 5667-16:1998 Water quality -- Sampling -- Part 16: Guidance on biotesting of samples
ISO 5814:1990 Water quality -- Determination of dissolved oxygen -- Electrochemical probe method
ISO 7346-1:1996 Water quality -- Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] -- Part 1: Static method
ISO 5667-16:1998 Water quality -- Sampling -- Part 16: Guidance on biotesting of samples
ISO 5814:1990 Water quality -- Determination of dissolved oxygen -- Electrochemical probe method
ISO 7346-1:1996 Water quality -- Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] -- Part 1: Static method
ISO 7346-2:1996 Water quality -- Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] -- Part 2: Semi-static method