Water quality. Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium. Method by analysis of inorganic carbon in sealed vessels (CO2 headspace test) (ISO 14593:1999)
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This International Standard specifies a method, by analysis of inorganic carbon, for the evaluation in an aqueous medium of the ultimate aerobic biodegradability of organic substances at a given concentration of microorganisms. This International Standard is applicable to organic compounds which are: a) water-soluble under the test conditions; b) poorly water-soluble under the test conditions, in which case special measures may be necessary to achieve a good dispersion of the compound (see ISO 10634); c) volatile; d) not inhibitory to the test microorganisms at the concentration chosen for the test. In this test, biogenically produced inorganic carbon is measured in situ in the test vessels in such a manner that the rate measured nearly equals the rate of microbial production. NOTE 1 The conditions described in this International Standard do not always correspond to the optimal conditions for allowing the maximum degree of biodegradation to occur. For alternative biodegradation methods see ISO 15462. NOTE 2 With highly volatile substances, losses to the gaseous phase can be minimized by reducing the volume of the headspace. However, there should be sufficient oxygen in the test system to prevent biodegradation being oxygen-limited. NOTE 3 The presence of an inhibitory effect can be determined as specified in 8.3, or by using any other method for determining the inhibitory effect of a substance on bacteria (see, for example, ISO 8192).
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Sisällysluettelo
Foreword
1 Scope
2 Normative reference
3 Terms and definitions
4 Principle
5 Test environment
6 Reagents
7 Apparatus
8 Procedure
8.1 Preparation of the test solutions
8.2 Preparation of the inoculum
8.3 Test procedure
8.4 Determination of total inorganic carbon (TIC)
9 Calculation and expression of results
9.1 Calculation
9.2 Acidification to pH < 3
9.3 Conversion of CO2 to carbonate
10 Expression of results
11 Validity of results
12 Precision of the method
13 Test report
Annex A Example of a biodegradation curve (informative)
Annex B Statistical treatment of results (informative)
ISO 10634:1995 Water quality -- Guidance for the preparation and treatment of poorly water-soluble organic compounds for the subsequent evaluation of their biodegradability in an aqueous medium
ISO 10634:1995 Water quality -- Guidance for the preparation and treatment of poorly water-soluble organic compounds for the subsequent evaluation of their biodegradability in an aqueous medium