Microbiology of food and animal feeding stuffs. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Requirements for sample preparation for qualitative detection (ISO 20837:2006)
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ISO 20837:2006 provides criteria and examples for sample preparation in order to obtain PCR-compatible samples or nucleic acids of suitable quality and quantity for PCR. It provides a description of the general principles involved. References to standards concerning the enrichment of microorganisms are given in Annex A, and a detailed method for DNA extraction is given in Annex B. ISO 20837:2006 has been established for food matrices, but could also be applied to feed and agricultural/environmental matrices with some adaptations, if necessary.
Tämän julkaisun valmistelusta Suomessa vastaa SFS Suomen Standardit, puh. 09 149 9331.
Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Principle
3.1 General
3.2 Enrichment and sample treatment
3.3 Nucleic acid extraction
4 General laboratory requirements
5 Reagents, apparatus and equipment
6 Procedure
6.1 Enrichment and sample treatment for bacteria
6.2 Nucleic acid extraction
6.3 Controls
Annex A Standards concerning the enrichment of microorganisms (bacteria) (informative)
Annex B Method for DNA extraction from Gram-negative bacteria (informative)
ISO 22174:2005 Microbiology of food and animal feeding stuffs -- Polymerase chain reaction (PCR) for the detection of food-borne pathogens -- General requirements and definitions
ISO 22174:2005 Microbiology of food and animal feeding stuffs -- Polymerase chain reaction (PCR) for the detection of food-borne pathogens -- General requirements and definitions