ISO 21567:2004 specifies a horizontal method for the detection of Shigella species. Subject to certain limitations, ISO 21567:2004 is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Enrichment in selective liquid medium
4.3 Plating out and identification of colonies
4.4 Biochemical and serological confirmation
5 Culture media, reagents and antisera
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure (see diagram in Annex A)
9.1 Test portion
9.2 Enrichment
9.3 Plating out and colony selection
9.4 Confirmation of colonies
9.5 Serological confirmation
10 Expression of results
11 Test report
Annex A Diagram of test procedure (normative)
Annex B Composition and preparation of culture media and reagents (normative)
Annex C Description of Shigella colony morphology and colour on selective agars, for both identification and quality control purposes (normative)
ISO 6887-1:1999 Microbiology of food and animal feeding stuffs -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 6887-2:2003 Microbiology of food and animal feeding stuffs -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 2: Specific rules for the preparation of meat and meat products
ISO 6887-3:2003 Microbiology of food and animal feeding stuffs -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 3: Specific rules for the preparation of fish and fishery products
ISO 6887-1:1999 Microbiology of food and animal feeding stuffs -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 6887-2:2003 Microbiology of food and animal feeding stuffs -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 2: Specific rules for the preparation of meat and meat products
ISO 6887-3:2003 Microbiology of food and animal feeding stuffs -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 3: Specific rules for the preparation of fish and fishery products
ISO 6887-4:2003 Microbiology of food and animal feeding stuffs -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 4: Specific rules for the preparation of products other than milk and milk products, meat and meat products, and fish and fishery products
ISO 7218:1996 Microbiology of food and animal feeding stuffs -- General rules for microbiological examinations
ISO 8261:2001 Milk and milk products -- General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination
ISO/TS 11133-1:2000 Microbiology of food and animal feeding stuffs -- Guidelines on preparation and production of culture media -- Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory
ISO/TS 11133-2:2003 Microbiology of food and animal feeding stuffs -- Guidelines on preparation and production of culture media -- Part 2: Practical guidelines on performance testing of culture media