Foodstuffs. Methods of analysis for the detection of genetically modified organisms and derived products. Quantitative nucleic acid based methods (ISO 21570:2005)
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ISO 21570:2005 provides the overall framework of quantitative methods for the detection of genetically modified organisms (GMO) in foodstuffs, using the polymerase chain reaction (PCR). It defines general requirements for the specific amplification of DNA target sequences in order to quantify the relative GMO-derived DNA content and to confirm the identity of the amplified DNA sequence. Guidelines, minimum requirements and performance criteria laid down in ISO 21570:2005 are intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories. ISO 21570:2005 has been established for food matrices, but is also applicable to other matrices, e.g. feed and plant samples from the environment.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Amplification, detection and confirmation of PCR products
4.3 Quantitation of PCR products
5 Reagents
6 Apparatus and equipment
7 Guidelines concerning the procedure
7.1 General
7.2 Target sequence stability
7.3 Calibration of the analysis
7.4 Quantitation considerations
7.5 Quality assurance requirements
8 Interpretation
9 Expression of results
10 Test report
Annex A Target taxon-specific methods (informative)
ISO 21569:2005 Foodstuffs -- Methods of analysis for the detection of genetically modified organisms and derived products -- Qualitative nucleic acid based methods
ISO 21571:2005 Foodstuffs -- Methods of analysis for the detection of genetically modified organisms and derived products -- Nucleic acid extraction
ISO 24276:2006 Foodstuffs -- Methods of analysis for the detection of genetically modified organisms and derived products -- General requirements and definitions
ISO 21569:2005 Foodstuffs -- Methods of analysis for the detection of genetically modified organisms and derived products -- Qualitative nucleic acid based methods
ISO 21571:2005 Foodstuffs -- Methods of analysis for the detection of genetically modified organisms and derived products -- Nucleic acid extraction
ISO 24276:2006 Foodstuffs -- Methods of analysis for the detection of genetically modified organisms and derived products -- General requirements and definitions
ISO Guide 32:1997 Calibration in analytical chemistry and use of certified reference materials