ISO 21571:2005 provides general requirements and specific methods for DNA extraction/purification and quantification. These methods are described in Annexes A and B. ISO 21571:2005 has been established for food matrices, but could also be applicable to other matrices, such as grains and feed. It has been designed as an integral part of nucleic-acid-based analytical methods, in particular ISO 21569 on qualitative analytical methods, and ISO 21570 on quantitative analytical methods.
Tämän julkaisun valmistelusta Suomessa vastaa SFS Suomen Standardit, puh. 09 149 9331.
Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Principle
3.1 General
3.2 DNA extraction
3.3 DNA quantitation
4 General laboratory requirements
5 Procedure
5.1 Preparation of the test portion
5.2 DNA extraction/purification
5.3 Quantitation of the extracted DNA
5.4 Stability of extracted DNA
6 Interpretation
7 Test report
Annex A Methods for DNA extraction (informative)
Annex B Methods for the quantitation of the extracted DNA (informative)
ISO 21569:2005 Foodstuffs -- Methods of analysis for the detection of genetically modified organisms and derived products -- Qualitative nucleic acid based methods
ISO 24276:2006 Foodstuffs -- Methods of analysis for the detection of genetically modified organisms and derived products -- General requirements and definitions
ISO 21569:2005 Foodstuffs -- Methods of analysis for the detection of genetically modified organisms and derived products -- Qualitative nucleic acid based methods
ISO 24276:2006 Foodstuffs -- Methods of analysis for the detection of genetically modified organisms and derived products -- General requirements and definitions