ISO 22160|IDF 209:2007 specifies a method for the determination of the alkaline phosphatase activity in pasteurized whole milk, semi-skimmed milk, skimmed milk, cream and flavoured milks using a chemiluminescent (EPAS) method. The method is applicable to milk and milk-based drinks from cows, sheep, buffalo and goats. The method is also suitable for any liquid-based sample if diluted in such a way that the diluted alkaline phosphatase activity has less than 7 000 milliunits per litre.
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Sisällysluettelo
Foreword
Foreword
1 Scope
2 Terms and definitions
3 Principle
4 Reagents
4.2 Stopping solution
4.4 Positive control
5 Apparatus
6 Sampling
7 Preparations
7.1 Test samples
7.2 Alkaline phosphatase-free milk
8 Procedure (see Annex A)
8.1 Calibration
8.2 Calibration adjustment
8.3 Control tests and calibration check
8.4 Determination
8.5 Heat-resistant microbial alkaline phosphatase control
9 Calculation and expression of results
9.1 General
9.2 Manual calculation
9.3 Expression of results
10 Precision
10.1 Interlaboratory test
10.2 Repeatability
10.3 Reproducibility
11 Test report
Annex A Procedure flowchart (informative)
Annex B Results of interlaboratory trials (informative)