Microbiology of food and animal feeding stuffs. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. General requirements and definitions (ISO 22174:2005)
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ISO 22174:2004 gives the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). It is applicable to the testing of foodstuffs and isolates obtained from foodstuffs for food-borne pathogens using the polymerase chain reaction (PCR) The minimum requirements laid down in ISO 22174:2004 are intended to ensure that comparable and reproducible results are obtained in different laboratories. ISO 22174:2004 has been established for food-borne pathogens in or isolated from food and feed matrices, but is also applicable to other matrices (e.g. environmental samples) and for the detection of non-pathogenic microorganisms.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
3.1 General terms
3.2 Terms related to the extraction and purification of DNA/RNA
3.3 Terms related to reverse transcription (RT) of RNA to DNA
3.4 Terms related to DNA amplification by PCR/RT-PCR
ISO 20837:2006 Microbiology of food and animal feeding stuffs -- Polymerase chain reaction (PCR) for the detection of food-borne pathogens -- Requirements for sample preparation for qualitative detection
ISO 20838:2006 Microbiology of food and animal feeding stuffs -- Polymerase chain reaction (PCR) for the detection of food-borne pathogens -- Requirements for amplification and detection for qualitative methods
ISO 3534-1:1993 Statistics -- Vocabulary and symbols -- Part 1: Probability and general statistical terms
ISO 20837:2006 Microbiology of food and animal feeding stuffs -- Polymerase chain reaction (PCR) for the detection of food-borne pathogens -- Requirements for sample preparation for qualitative detection
ISO 20838:2006 Microbiology of food and animal feeding stuffs -- Polymerase chain reaction (PCR) for the detection of food-borne pathogens -- Requirements for amplification and detection for qualitative methods
ISO 3534-1:1993 Statistics -- Vocabulary and symbols -- Part 1: Probability and general statistical terms
ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results -- Part 1: General principles and definitions
ISO/IEC 17025:1999 General requirements for the competence of testing and calibration laboratories