ISO 20963:2005 describes a method for the determination of the effects of contaminated soils and substances on the survival of the larvae of Oxythyrea funesta. The larvae are exposed to the pollutants by cuticular and alimentary uptake. For contaminated soils, the effects on the survival are determined in the test soil and in a control soil. Depending on the objectives of the study, the control and dilution substrates (dilution series of contaminated soil) are either uncontaminated soil comparable to the soil sample to be tested or artificial soil substrate. Effects of substances are assessed using a defined artificial soil substrate. ISO 20963:2005 is not applicable to volatile substances, i.e. substances for which Henry's constant or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,001 33 Pa at 25 °C. This method does not take into account the possible degradation of the substances or pollutants during the test.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test environment
6 Reagents
6.1 Biological material
6.2 Test substrate
7 Apparatus
8 Procedure
8.1 Test design
8.2 Preparation of test mixture
8.3 Preparation of control container
8.4 Food addition
8.5 Introduction of the biological material
8.6 Test conditions and measurements
8.7 Reference substance
9 Expression of results
9.1 Calculation
9.2 Expression of results
10 Validity of the test
11 Test report
Annex A Example of breeding technique for Oxythyrea funesta (informative)
Annex B Results of the collaborative trial carried out in France (informative)
ISO 10381-6:2009 Soil quality -- Sampling -- Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 10390:2005 Soil quality -- Determination of pH
ISO 11268-1:1993 Soil quality -- Effects of pollutants on earthworms (Eisenia fetida) -- Part 1: Determination of acute toxicity using artificial soil substrate
ISO 10381-6:2009 Soil quality -- Sampling -- Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 10390:2005 Soil quality -- Determination of pH
ISO 11268-1:1993 Soil quality -- Effects of pollutants on earthworms (Eisenia fetida) -- Part 1: Determination of acute toxicity using artificial soil substrate
ISO 11269-2:2005 Soil quality -- Determination of the effects of pollutants on soil flora -- Part 2: Effects of chemicals on the emergence and growth of higher plants