ISO 22030:2005 describes a method for determining the inhibition of the growth and reproductive capability of higher plants by soils under controlled conditions. Two species are recommended: a rapid-cycling variant of turnip rape (Brassica rapa CrGC syn. Rbr) and oat (Avena sativa). The duration of test should be sufficient to include chronic endpoints that demonstrate the reproductive capability of the test plants. By using natural test soils, e.g. from contaminated sites or remediated soils, and by comparing the development of the test plants in these soils with reference or standard control soils, the test is applicable to assess soil quality, especially the function of the soil as a habitat for plants. This method can be modified to allow use of the chronic plant assay for the testing of chemicals incorporated into soil. By preparing a dilution series of a test substance in standard control soils, the same endpoints can be measured to assess the chronic toxicity of chemicals. This method is not applicable to volatile substances, i.e. substances for which Henry's constant or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Materials
5.1 Test plants
5.2 Soil and soil storage
5.3 Reference substance
6 Apparatus
6.5 Test vessels
7 Methods
7.1 Experimental design
7.2 Pre-test
7.3 Preliminary test (range-finding)
7.4 Final test
7.5 Preparation of the pots
7.6 Preparation of the seeds
7.7 Growth conditions
7.8 Start of the test
7.9 Handling during the test
7.10 Endpoint measurements
7.11 Summary and timetable of the test
8 Validity criteria
9 Assessment of the results
9.1 Presentation of measured data
9.2 Statistical analysis
10 Test report
Annex A Testing of chemicals in soil (informative)
ASTM D 1076:2002 Standard Specification for Rubber-Concentrated, Ammonia Preserved, Creamed, and Centrifuged Natural Latex
ISO 11268-1:1993 Soil quality -- Effects of pollutants on earthworms (Eisenia fetida) -- Part 1: Determination of acute toxicity using artificial soil substrate
ISO 11268-2:1998 Soil quality -- Effects of pollutants on earthworms (Eisenia fetida) -- Part 2: Determination of effects on reproduction
ASTM D 1076:2002 Standard Specification for Rubber-Concentrated, Ammonia Preserved, Creamed, and Centrifuged Natural Latex
ISO 11268-1:1993 Soil quality -- Effects of pollutants on earthworms (Eisenia fetida) -- Part 1: Determination of acute toxicity using artificial soil substrate
ISO 11268-2:1998 Soil quality -- Effects of pollutants on earthworms (Eisenia fetida) -- Part 2: Determination of effects on reproduction
ISO 11269-2:2005 Soil quality -- Determination of the effects of pollutants on soil flora -- Part 2: Effects of chemicals on the emergence and growth of higher plants
ISO 15176:2002 Soil quality -- Characterization of excavated soil and other soil materials intended for re-use
ISO 15799:2003 Soil quality -- Guidance on the ecotoxicological characterization of soils and soil materials