Microbiology of food and animal feeding stuffs. Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens. General requirements and definitions (ISO 22119:2011)
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ISO 22119:2010 defines terms for the detection of food-borne pathogens in foodstuffs, and isolates obtained from them, using the polymerase chain reaction (PCR). ISO 22119:2010 specifies requirements for the amplification and detection of nucleic acid sequences (DNA or RNA after reverse transcription) by real-time PCR. The minimum requirements laid down in ISO 22119:2010 provide the basis for comparable and reproducible results within individual and between different laboratories. ISO 22119:2010 is also applicable, for example, to the detection of food-borne pathogens in environmental samples and in animal feeding stuffs.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Probes for real-time PCR
5 General laboratory requirements
6 Reagents and materials
6.1 General
6.2 DNA polymerase and reaction buffer
6.3 Deoxyribonucleoside triphosphates (dNTPs) for PCR
ISO 20838:2006 Microbiology of food and animal feeding stuffs -- Polymerase chain reaction (PCR) for the detection of food-borne pathogens -- Requirements for amplification and detection for qualitative methods
ISO 22174:2005 Microbiology of food and animal feeding stuffs -- Polymerase chain reaction (PCR) for the detection of food-borne pathogens -- General requirements and definitions
ISO 20838:2006 Microbiology of food and animal feeding stuffs -- Polymerase chain reaction (PCR) for the detection of food-borne pathogens -- Requirements for amplification and detection for qualitative methods
ISO 22174:2005 Microbiology of food and animal feeding stuffs -- Polymerase chain reaction (PCR) for the detection of food-borne pathogens -- General requirements and definitions