Water quality. Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium by determination of oxygen demand in a closed respirometer (ISO 9408:1999)
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1 ScopeThis International Standard specifies a method, by determination of the oxygen demand in a closed respirometer, forthe evaluation in aqueous medium of the ultimate biodegradability of organic compounds and waste waters at a givenconcentration by aerobic microorganisms.The method applies to organic compounds whicha) are water-soluble under the conditions of the test;b) are poorly water-soluble under the conditions of the test, in which case special measures may be necessary toachieve good dispersion of the compound (see for example, ISO 10634);c) do not reach and react with the CO2 absorbent;d) are volatile, provided that a suitable respirometer or suitable conditions (e.g. a smaller ratio of volume headspace to volume liquid medium) are used;e) are not inhibitory to the test microorganisms at the concentration chosen for the test. The presence of inhibitoryeffects can be determined as specified in 7.3, or by using any other method for determining the inhibitory effect ofa compound on bacteria (see, for example, ISO 8192).NOTE The conditions described in this International Standard do not always correspond to the optimal conditions forallowing the maximum degree of biodegradation to occur. For alternative biodegradation methods, see ISO 15462.
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Sisällysluettelo
Foreword
1 Scope
2 Terms and definitions
3 Principle
4 Test environment
5 Reagents
5.1 Water
5.2 Test medium
5.3 Carbon dioxide absorber
5.4 Mercury chloride solution
5.5 Sodium hydroxide solution
5.6 Hydrochloric acid solution
6 Apparatus
6.1 Closed respirometer
6.2 Water-bath or constant temperature room (to comply with clause 4)
6.3 Equipment for measurement of dissolved organic carbon
6.4 Device for determining chemical oxygen demand (COD) (optional)
6.5 Centrifuge or device for filtration
6.6 pH meter (usual laboratory equipment)
7 Procedure
7.1 Preparation of the test solutions
7.2 Preparation of the inoculum
7.3 Test
8 Calculation and expression of results
8.1 Calculation
8.2 Expression of results
9 Validity of results
9.1 Validity criteria
9.2 Inhibition
9.3 pH value
10 Test report
Annex A Example of calculation of theoretical oxygen demand (informative)
Annex B Correction of oxygen uptake for interference by nitrification (informative)
Annex С Example of a biodegradation curve (informative)