ISO 29822:2009 specifies the determination of the degree of isomerization of diacylglycerols in vegetable fats and oils. 1,2-Diacylglycerols are transformed to the more stable 1,3-isomers during storage or due to acidic catalysed reaction. The mass fraction of 1,2-diacylglycerols can be used as a quality criterion for vegetable fats and oils.
Tämän julkaisun valmistelusta Suomessa vastaa SFS Suomen Standardit, puh. 09 149 9331.
Sisällysluettelo
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sample
7.1 Sampling
7.2 Preparation of test sample
8 Procedure
8.1 Preparation of the silica gel chromatography column
8.2 Separation of the fraction containing non polar lipids
8.3 Preparation of trimethylsilyl ethers (silylation)
8.4 Gas chromatography
8.5 Identification of diacylglycerol isomers
9 Result of the determination
9.1 Identification of diacylglycerols
9.2 Determination of the peak areas of 1,2- and 1,3- diacylglycerols in the oil
10 Precision of the method
10.1 Interlaboratory test
10.2 Repeatability
10.3 Reproducibility
11 Test report
Annex A Example of a typical chromatogram (informative)
Annex B Results of an interlaboratory test (informative)