1 Scope This part of ISO 11268 specifies one of the methods for evaluating the habitat function of soils and determining the acute toxicity of soil contaminants and chemicals to Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. It is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials. Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects on survival are determined in the test soil and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) should be either an uncontaminated soil comparable to the soil sample to be tested (reference soil) or a standard soil (e.g. artificial soil). Information is provided on how to use this method for testing chemicals under temperate as well as under tropical conditions. The method is not applicable to volatile substances, i.e. substances for which H (Henry's constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C. This method does not take into account the possible degradation of the substances or contaminants during the test.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and material
6 Apparatus
7 Procedure
7.1 Experimental design
7.2 Preparation of test mixture
7.3 Addition of the earthworms
7.4 Test conditions and measurements
7.5 Reference substance
8 Calculation and expression of results
8.1 Calculation
8.2 Expression of results
9 Validity of the test
10 Statistical analysis
10.1 General
10.2 Single-concentration tests
10.3 Multi-concentration tests
11 Test report
Annex A Determination of the acute toxicity of chemicals (in particular pesticides) on earthworms under tropical test conditions (informative)
Annex B Culturing of Eisenia fetida and Eisenia andrei (informative)
Annex C Determination of water holding capacity of artificial soil (informative)
Annex D Background information on the acute effect of boric acid on earthworms (informative)
ISO 10381-6:2009 Soil quality -- Sampling -- Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 10390:2005 Soil quality -- Determination of pH
ISO 10694:1995 Soil quality -- Determination of organic and total carbon after dry combustion (elementary analysis)
ISO 10381-6:2009 Soil quality -- Sampling -- Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 10390:2005 Soil quality -- Determination of pH
ISO 10694:1995 Soil quality -- Determination of organic and total carbon after dry combustion (elementary analysis)
ISO 11260:1994 Soil quality -- Determination of effective cation exchange capacity and base saturation level using barium chloride solution
ISO 11277:2009 Soil quality -- Determination of particle size distribution in mineral soil material -- Method by sieving and sedimentation
ISO 11465:1993 Soil quality -- Determination of dry matter and water content on a mass basis -- Gravimetric method