ISO 18743:2015 specifies a method of detection of Trichinella spp. muscle stage larvae in meat of individual animal carcasses intended for human consumption. It is applicable to the examination of meat from domestic and sylvatic animal species, which can be infected by nematodes of the genus Trichinella. This method does not allow the identification of the species or genotype of detected parasites; species or genotype identification can be carried out by molecular methods. The method described in this International Standard is intended to be used in conjunction with the guidelines in the OIE Manual of Diagnostic Tests and Vaccines and by the International Commission on Trichinellosis (ICT) for Trichinella testing and the inspection of carcasses intended for human consumption, unless it has been demonstrated by other means that the animal was not at risk for exposure to Trichinella. The artificial digestion/magnetic stirrer method is considered to be the standard method because it has proven to give the most reliable results in validation studies. NOTE Provided equivalence with the method described within this International Standard can be documented, alternative methods can be used for analysis.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Sample size
4.3 Blending/grinding of the muscle sample
4.4 Preparation of the digest fluid
4.5 Digestion of the chopped meat
4.6 Filtration of the digest fluid
4.7 Sedimentation of the digest fluid
4.8 Microscopic examination
4.9 Verification of findings
5 Reagents
6 Apparatus
7 Sampling, labelling, and transport
8 Sample preparation
9 Procedure
9.1 General
9.2 Blending/grinding
9.3 Preparation of the digest fluid
9.4 Digestion of the chopped meat in the glass beaker
9.5 Filtration of the digest fluid
9.6 Sedimentation of the digest fluid in the separatory funnel
9.7 Collection of the primary and secondary sediment
9.8 Microscopic examination
10 Documentation
11 Expression of the results
12 Safety measures
Annex A Sample collection (normative)
Annex B Frozen samples (normative)
Annex C Artificial digestion magnetic stirrer method (informative)
Annex D Example of a laboratory worksheet for recording data when testing pooled samples by digestion assay (informative)