ISO 11702:2016 specifies a method for the quantitative determination of the total sterols content by means of an enzymatic staining test. The method is applicable to free and esterified sterols in animal and vegetable fats and oils, fatty foods and related products. The determination is applicable to sample quantities of 1 g to 2 g of fat. The method is not applicable to dark coloured fats and oils. The enzyme is not specific for cholesterol, but also oxidizes other 3-hydroxysterols. The method has not been tested for products fortified with sterols at higher levels. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of ISO 11702:2016.
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Sisällysluettelo
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Preparation of the test sample
9 Procedure
10 Result of the determination
11 Precision of the method
11.1 Interlaboratory test
11.2 Repeatability limit
11.3 Reproducibility limit
12 Test report
Annex A Results of an interlaboratory test (informative)
ISO 3696:1987 Water for analytical laboratory use -- Specification and test methods
ISO 661:2003 Animal and vegetable fats and oils -- Preparation of test sample
ISO 3696:1987 Water for analytical laboratory use -- Specification and test methods
ISO 661:2003 Animal and vegetable fats and oils -- Preparation of test sample