ISO 11731:2017 specifies culture methods for the isolation of Legionella and estimation of their numbers in water samples. These methods are applicable to all kinds of water samples including potable, industrial, waste and natural waters. These methods can be used for water related matrices, e.g. biofilms, sediments, etc. Not all Legionella species are culturable; therefore, the methods described in this document do not recover all species of Legionella.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Examination
4.3 Confirmation
5 Apparatus and glassware
6 Culture media and reagents
7 Sampling
8 Procedure
8.1 Samples
8.2 Concentration of water samples
8.3 Sample pre-treatment
8.4 Culture
8.5 Confirmation of presumptive Legionella colonies on culture media: BCYE agar and BCYE–cys agar
9 Expression of results
10 Test report
11 Quality assurance
11.1 General
11.2 Performance testing of Legionella culture media
11.3 Preparing working culture and test suspension for performance testing
Annex A Legionella species (informative)
Annex B Culture media (normative)
Annex C Diluents (normative)
Annex D Acid solution (normative)
Annex E Scraping or rubbing the bacteria from membrane filters (informative)
Annex F Centrifugation technique (informative)
Annex G Indirect immunofluorescent antibody assay for the identification of Legionella species (informative)
Annex H Performance data (informative)
Annex I Pre-treatment of water related matrices (informative)
ISO 11133:2014 Microbiology of food, animal feed and water -- Preparation, production, storage and performance testing of culture media
ISO 19458:2006 Water quality -- Sampling for microbiological analysis
ISO 3696:1987 Water for analytical laboratory use -- Specification and test methods
ISO 11133:2014 Microbiology of food, animal feed and water -- Preparation, production, storage and performance testing of culture media
ISO 19458:2006 Water quality -- Sampling for microbiological analysis
ISO 3696:1987 Water for analytical laboratory use -- Specification and test methods
ISO 7704:1985 Water quality -- Evaluation of membrane filters used for microbiological analyses
ISO 8199:2005 Water quality -- General guidance on the enumeration of micro-organisms by culture