Microbiology of the food chain. Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp.. Part 1: Detection method (ISO 11290-1:2017)
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ISO 11290-1:2017 specifies a horizontal method for - the detection of L. monocytogenes, and - the detection of Listeria spp. (including L. monocytogenes). ISO 11290-1:2017 is applicable to - products intended for human consumption and for the feeding of animals, and - environmental samples in the area of food production and food handling.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Primary enrichment in a selective liquid enrichment medium with reduced concentration of selective agents (half-Fraser broth)
4.3 Secondary enrichment with a selective liquid enrichment medium with full concentration of selective agents (Fraser broth)
4.4 Plating out and identification
4.5 Confirmation
5 Culture media and reagents
6 Equipment and consumables
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion and initial suspension
9.2 Primary enrichment
9.3 Secondary enrichment
9.4 Plating out and identification
9.5 Confirmation of Listeria monocytogenes or Listeria spp.
9.6 Interpretation of morphological and physiological properties and of the biochemical reactions
9.7 Additional characterization of isolated strains (optional)
10 Expression of results
11 Performance characteristics of the method
11.1 Method validation studies
11.2 Sensitivity
11.3 Specificity
11.4 Level of detection (LOD50)
12 Test report
13 Quality assurance
Annex A Diagram of procedure (normative)
Annex B Composition and preparation of culture media and reagents (normative)
Annex C Distinction of Listeria spp. from other genera (informative)
Annex D Reactions for the identification of Listeria species (informative)
Annex E Listeria selective agars (informative)
Annex F Results of the interlaboratory studies for detection of Listeria monocytogenes (informative)
ISO 11133:2014 Microbiology of food, animal feed and water -- Preparation, production, storage and performance testing of culture media
ISO 6887-1:2017 Microbiology of the food chain -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 6887-2:2017 Microbiology of the food chain -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 2: Specific rules for the preparation of meat and meat products
ISO 11133:2014 Microbiology of food, animal feed and water -- Preparation, production, storage and performance testing of culture media
ISO 6887-1:2017 Microbiology of the food chain -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 6887-2:2017 Microbiology of the food chain -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 2: Specific rules for the preparation of meat and meat products
ISO 6887-3:2017 Microbiology of the food chain -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 3: Specific rules for the preparation of fish and fishery products
ISO 6887-4:2017 Microbiology of the food chain -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 4: Specific rules for the preparation of miscellaneous products
ISO 6887-5:2010 Microbiology of food and animal feeding stuffs -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 5: Specific rules for the preparation of milk and milk products
ISO 6887-6:2013 Microbiology of food and animal feed -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 6: Specific rules for the preparation of samples taken at the primary production stage
ISO 7218:2007 Microbiology of food and animal feeding stuffs -- General requirements and guidance for microbiological examinations
882/2004 Rehu- ja elintarvikelainsäädännön sekä eläinten terveyttä ja hyvinvointia koskevien sääntöjen mukaisuuden varmistamiseksi suoritetusta virallisesta valvonnasta