Microbiology of the food chain. Horizontal method for the detection and enumeration of Enterobacteriaceae. Part 2: Colony-count technique (ISO 21528-2:2017, Corrected version 2018-06-01)
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ISO 21528-2:2017 specifies a method for the enumeration of Enterobacteriaceae. It is applicable to - products intended for human consumption and the feeding of animals, and - environmental samples in the area of primary production, food production and food handling. This technique is intended to be used when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample. The most probable number (MPN) technique, as included in ISO 21528-1, is generally used when the number sought is expected to be below 100 per millilitre or per gram of test sample.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Preparation of initial suspension and decimal dilutions
4.2 Isolation and selection for confirmation
4.3 Confirmation
4.4 Calculation
5 Diluent, culture media and reagent
6 Equipment and consumables
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 General
9.2 Test portion, initial suspension and dilutions
9.3 Inoculation and incubation
9.4 Counting and selection of colonies for confirmation
9.5 Subculturing selected colonies
9.6 Biochemical confirmation tests
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory study
11.2 Repeatability limit
11.3 Reproducibility limit
12 Test report
13 Quality assurance
Annex A Culture media and reagents (normative)
Annex B Method validation studies and performance characteristics (informative)
ISO 11133:2014 Microbiology of food, animal feed and water -- Preparation, production, storage and performance testing of culture media
ISO 18593:2018 Microbiology of the food chain -- Horizontal methods for surface sampling
ISO 6887-1:2017 Microbiology of the food chain -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 11133:2014 Microbiology of food, animal feed and water -- Preparation, production, storage and performance testing of culture media
ISO 18593:2018 Microbiology of the food chain -- Horizontal methods for surface sampling
ISO 6887-1:2017 Microbiology of the food chain -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 6887-2:2017 Microbiology of the food chain -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 2: Specific rules for the preparation of meat and meat products
ISO 6887-3:2017 Microbiology of the food chain -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 3: Specific rules for the preparation of fish and fishery products
ISO 6887-4:2017 Microbiology of the food chain -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 4: Specific rules for the preparation of miscellaneous products
ISO 6887-5:2010 Microbiology of food and animal feeding stuffs -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 5: Specific rules for the preparation of milk and milk products
ISO 6887-6:2013 Microbiology of food and animal feed -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 6: Specific rules for the preparation of samples taken at the primary production stage
ISO 7218:2007 Microbiology of food and animal feeding stuffs -- General requirements and guidance for microbiological examinations
882/2004 Rehu- ja elintarvikelainsäädännön sekä eläinten terveyttä ja hyvinvointia koskevien sääntöjen mukaisuuden varmistamiseksi suoritetusta virallisesta valvonnasta