Water quality. Determination of the growth inhibition effects of waste waters, natural waters and chemicals on the duckweed Spirodela polyrhiza. Method using a stock culture independent microbiotest (ISO 20227:2017)
Tuote ladattavissa hetiToimitusaika on noin 1 - 2 työpäivääToimitusaika on 3 - 5 työpäivää.
ISO 20227:2017 specifies a method for the determination of the inhibition of the growth of the first fronds of Spirodela polyrhiza germinated from turions, by substances and mixtures contained in water or waste water, including treated municipal waste water and industrial effluents. The test is also applicable to pure chemicals and in particular, plant protection products and pesticides.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test organisms
6 Growth medium
6.1 Preparation of stock solutions
6.2 Preparation of the final concentration of modified Steinberg medium
7 Apparatus
8 Reference chemicals
9 Procedure
9.1 Germination of the Spirodela polyrhiza turions
9.2 Tests on effluents (and waste waters)
9.3 Tests on chemical compounds
9.4 Filling of the test plate with the toxicant dilutions
9.5 Transfer of the germinated turions in the test cups
9.6 Photo of the multiwell at the start of the toxicity test
9.7 Incubation of the multiwell
9.8 Photo of the multiwell at the end of the toxicity test
9.9 Measurement of the area of the first fronds
10 Data treatment — Calculation of the growth inhibition
11 Validity criterion
12 Test sensitivity
13 Test with reference chemicals
14 Test report
Annex A Spirodela polyrhiza stock culturing for turion production (informative)
Annex B Sensitivity of the Spirodela polyrhiza microbiotest (informative)