Microbiology of the food chain. Horizontal method for the determination of Vibrio spp.. Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus (ISO 21872-1:2017)
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ISO 21872-1:2017 specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus. ISO 21872-1:2017 is applicable to the following: - products intended for human consumption and the feeding of animals; - environmental samples in the area of food production and food handling.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Primary enrichment in a liquid selective medium
4.3 Secondary enrichment in a liquid selective medium
4.4 Isolation and identification
4.5 Confirmation
5 Culture media and reagents
5.1 Enrichment medium: alkaline saline peptone water (ASPW)
5.2 Solid selective isolation media
5.3 Saline nutrient agar (SNA)
5.4 Reagent for detection of oxidase
5.5 Biochemical tests
5.6 PCR
6 Equipment and consumables
7 Sampling
8 Preparation of the test sample
9 Procedure (See Figure A.1)
9.1 Test portion and initial suspension
9.2 Primary selective enrichment
9.3 Secondary selective enrichment
9.4 Isolation and identification
9.5 Confirmation
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory study
11.2 Sensitivity
11.3 Specificity
11.4 LOD50
12 Test report
Annex A Diagram of procedure (normative)
Annex B Composition and preparation of the culture media and reagents (normative)
Annex C Conventional PCR for the detection of Vibrio parahaemolyticus, thermostable direct haemolysin (tdh) and thermostable direct related haemolysin (trh) genes, Vibrio cholerae and Vibrio vulnificus (informative)
Annex D Real-time PCR for the detection of Vibrio parahaemolyticus, thermostable direct haemolysin gene (tdh) and Vibrio vulnificus (informative)
Annex E Results of an interlaboratory study (informative)
4.2 Primary enrichment in a liquid selective medium
4.3 Secondary enrichment in a liquid selective medium
4.4 Isolation and identification
4.5 Confirmation
5 Culture media and reagents
5.1 Enrichment medium: alkaline saline peptone water (ASPW)
5.2 Solid selective isolation media
5.3 Saline nutrient agar (SNA)
5.4 Reagent for detection of oxidase
5.5 Biochemical tests
5.6 PCR
6 Equipment and consumables
7 Sampling
8 Preparation of the test sample
9 Procedure (See Figure A.1)
9.1 Test portion and initial suspension
9.2 Primary selective enrichment
9.3 Secondary selective enrichment
9.4 Isolation and identification
9.5 Confirmation
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory study
11.2 Sensitivity
11.3 Specificity
11.4 LOD50
12 Test report
Annex A Diagram of procedure (normative)
Annex B Composition and preparation of the culture media and reagents (normative)
Annex C Conventional PCR for the detection of Vibrio parahaemolyticus, thermostable direct haemolysin (tdh) and thermostable direct related haemolysin (trh) genes, Vibrio cholerae and Vibrio vulnificus (informative)
Annex D Real-time PCR for the detection of Vibrio parahaemolyticus, thermostable direct haemolysin gene (tdh) and Vibrio vulnificus (informative)
Annex E Results of an interlaboratory study (informative)
ISO 11133:2014 Microbiology of food, animal feed and water -- Preparation, production, storage and performance testing of culture media
ISO 22118:2011 Microbiology of food and animal feeding stuffs -- Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens -- Performance characteristics
ISO 22119:2011 Microbiology of food and animal feeding stuffs -- Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens -- General requirements and definitions
ISO 11133:2014 Microbiology of food, animal feed and water -- Preparation, production, storage and performance testing of culture media
ISO 22118:2011 Microbiology of food and animal feeding stuffs -- Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens -- Performance characteristics
ISO 22119:2011 Microbiology of food and animal feeding stuffs -- Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens -- General requirements and definitions
ISO 22174:2005 Microbiology of food and animal feeding stuffs -- Polymerase chain reaction (PCR) for the detection of food-borne pathogens -- General requirements and definitions
ISO 6887-1:2017 Microbiology of the food chain -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 6887-3:2017 Microbiology of the food chain -- Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 3: Specific rules for the preparation of fish and fishery products
ISO 7218:2007 Microbiology of food and animal feeding stuffs -- General requirements and guidance for microbiological examinations
882/2004 Rehu- ja elintarvikelainsäädännön sekä eläinten terveyttä ja hyvinvointia koskevien sääntöjen mukaisuuden varmistamiseksi suoritetusta virallisesta valvonnasta