Soil quality. Avoidance test for determining the quality of soils and effects of chemicals on behaviour. Part 1: Test with earthworms (Eisenia fetida and Eisenia andrei) (ISO 17512-1:2008)
Tuote ladattavissa hetiToimitusaika on noin 1 - 2 työpäivääToimitusaika on 3 - 5 työpäivää.
ISO 17512-1:2008 specifies a rapid screening method for evaluating the habitat function of soils and the influence of contaminants and chemicals on earthworm behaviour. The sublethal test is a rapid method that reflects the bioavailability of contaminant mixtures in natural soils and substances spiked into soils to Eisenia fetida and Eisenia andrei. The avoidance behaviour of the worms is the measurement endpoint of the test. This test is not intended to replace the earthworm reproduction test. Two different designs (a two section unit and a six section unit) have been developed and successfully applied. Both designs are applicable to either single-concentration (e. g. for assessing the quality of a field soil) or multi-concentration (e. g. for assessing the toxicity of a spiked chemical) tests. In both cases, the earthworms are allowed to make the initial choice on which compartment, control and a treatment [in the two section test vessel between right and left side; in the six section test vessel between the (3 + 3) alternating compartments], to enter.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and materials
6 Apparatus
6.1 Containers (see Annex A).
7 Procedure
7.1 Appropriate concentration range
7.2 Testing of soils
7.3 Testing of chemical
7.4 Reference substance
7.5 Validity criteria
8 Calculation and expression of results
9 Test report
Annex A Test chambers (informative)
Annex B Example of a breeding technique for Eisenia fetida and Eisenia andrei (informative)
Annex C Further test organisms (informative)
Annex D Contaminants that earthworms can detect and avoid in the avoidance test (informative)
Annex E Testing of chemicals in the avoidance test (normative)
Annex F Determination of water-holding capacity (normative)
Annex G Comparison of the results obtained in the two section chamber and six section chamber system (informative)
Annex H Influence of soil properties on avoidance behaviour — Basis for the threshold value of 20 % (informative)
Annex I Data gained in “dual” tests with the same (untreated) control soil on both sides of the test vessels (informative)
ISO 10390:2005 Soil quality -- Determination of pH
ISO 11268-2:1998 Soil quality -- Effects of pollutants on earthworms (Eisenia fetida) -- Part 2: Determination of effects on reproduction
ISO 11269-2:2012 Soil quality -- Determination of the effects of pollutants on soil flora -- Part 2: Effects of contaminated soil on the emergence and early growth of higher plants
ISO 10390:2005 Soil quality -- Determination of pH
ISO 11268-2:1998 Soil quality -- Effects of pollutants on earthworms (Eisenia fetida) -- Part 2: Determination of effects on reproduction
ISO 11269-2:2012 Soil quality -- Determination of the effects of pollutants on soil flora -- Part 2: Effects of contaminated soil on the emergence and early growth of higher plants
ISO 11465:1993 Soil quality -- Determination of dry matter and water content on a mass basis -- Gravimetric method
ISO 15799:2019 Soil quality -- Guidance on the ecotoxicological characterization of soils and soil materials