Soil quality. Avoidance test for determining the quality of soils and effects of chemicals on behaviour. Part 2: Test with collembolans (Folsomia candida) (ISO 17512-2:2011)
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ISO 17512-2:2011 specifies a rapid screening method for evaluating the habitat function of soils based on the avoidance behaviour of springtails. The test is a rapid method that reflects the bioavailability of contaminants in natural soils and substances spiked into soils to Folsomia candida. In both cases, it is possible to establish a dose-response-relationship. The avoidance behaviour of the springtails is the measurement endpoint of the test. This test is not intended to replace the Collembola reproduction test.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and materials
6 Apparatus
7 Procedure
7.1 Appropriate concentration/dilution range
7.2 Testing of soils
7.3 Reference substance
7.4 Validity criteria
8 Calculation and expression of results
8.1 General
8.2 Calculation of the percentage of avoidance
8.3 NOEC/LOEC calculation
8.4 ECx calculation
9 Test report
Annex A Techniques for rearing and breeding of Collembola (informative)
Annex B Test chambers (informative)
Annex C Testing of chemicals in the avoidance test (normative)
Annex D Determination of the water-holding capacity (normative)
ISO 10390:2005 Soil quality -- Determination of pH
ISO 11267:1999 Soil quality -- Inhibition of reproduction of Collembola (Folsomia candida) by soil pollutants
ISO 11268-2:1998 Soil quality -- Effects of pollutants on earthworms (Eisenia fetida) -- Part 2: Determination of effects on reproduction
ISO 10390:2005 Soil quality -- Determination of pH
ISO 11267:1999 Soil quality -- Inhibition of reproduction of Collembola (Folsomia candida) by soil pollutants
ISO 11268-2:1998 Soil quality -- Effects of pollutants on earthworms (Eisenia fetida) -- Part 2: Determination of effects on reproduction
ISO 11269-2:2012 Soil quality -- Determination of the effects of pollutants on soil flora -- Part 2: Effects of contaminated soil on the emergence and early growth of higher plants
ISO 11465:1993 Soil quality -- Determination of dry matter and water content on a mass basis -- Gravimetric method
ISO 15799:2019 Soil quality -- Guidance on the ecotoxicological characterization of soils and soil materials