The purpose of ISO 29200:2013 is to describe a method for assessing genotoxic effects (chromosome breakage or dysfunction of the mitotic spindle) of soils or soil materials on the secondary roots of a higher plant: Vicia faba (broad bean). This method allows the assessment of genotoxicity (toxicity for genetic material) of soils and soil materials like compost, sludge, waste, fertilizing matters, etc. Two ways of exposure can be considered: a direct exposure of plants to the soil (or soil material) which is relevant for the real genotoxic potential and an exposure of plants to the water extract of the soil (or soil material). This last way of exposure to a leachate or an eluate allows the detection of the mutagens which are not adsorbed to soils and which may be transferred to aquatic compartments. Moreover, this test may be used to evaluate genotoxic effects of chemical substances and to waters, effluents, etc.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative reference
3 Terms and definitions
4 Principle
5 Plants, test equipment and reagents
5.1 Equipment
5.2 Test organism
5.3 Reference substance
5.4 Reagents
6 Protocols
6.1 Preparation of the soil to be tested
6.2 Preparation of the seeds
6.3 Conducting of the test
6.4 Test environment
6.5 Cell preparation
7 Assessment of the results
7.1 Presentation of the data
7.2 Statistical analysis
7.3 Interpretation of the results
8 Validity criteria
9 Test report
Annex A Composition of Hoagland's medium (informative)
Annex B Testing chemicals added to soils (informative)
Annex C Results of the interlaboratory test conducted within the framework of NF T 90-327 (informative)
Annex D Results of the interlaboratory test conducted on the reference substance and an industrial contaminated soil (informative)
EN 14735:2005 Characterization of waste - Preparation of waste samples for ecotoxicity tests
EN 14735:2021 Characterization of waste - Preparation of waste samples for ecotoxicity tests
ISO 10381-6:2009 Soil quality -- Sampling -- Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
EN 14735:2005 Characterization of waste - Preparation of waste samples for ecotoxicity tests
EN 14735:2021 Characterization of waste - Preparation of waste samples for ecotoxicity tests
ISO 10381-6:2009 Soil quality -- Sampling -- Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 10390:2005 Soil quality -- Determination of pH
ISO 10694:1995 Soil quality -- Determination of organic and total carbon after dry combustion (elementary analysis)
ISO 11260:2018 Soil quality -- Determination of effective cation exchange capacity and base saturation level using barium chloride solution
ISO 11269-2:2012 Soil quality -- Determination of the effects of pollutants on soil flora -- Part 2: Effects of contaminated soil on the emergence and early growth of higher plants
ISO 11465:1993 Soil quality -- Determination of dry matter and water content on a mass basis -- Gravimetric method
ISO/TS 21268-1:2007 Soil quality -- Leaching procedures for subsequent chemical and ecotoxicological testing of soil and soil materials -- Part 1: Batch test using a liquid to solid ratio of 2 l/kg dry matter
ISO/TS 21268-2:2007 Soil quality -- Leaching procedures for subsequent chemical and ecotoxicological testing of soil and soil materials -- Part 2: Batch test using a liquid to solid ratio of 10 l/kg dry matter