Verkkokauppa
SFS-EN ISO 11063:2020:en
Soil quality. Direct extraction of soil DNA (ISO 11063:2020)
Soveltamisala
Suomenkielistä soveltamisalaa ei ole saatavissa.
The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.
The direct extraction of DNA from soil samples provides unique insight into the a- and ß-diversity of microbial communities. Next-generation sequencing of amplicons obtained by PCR (polymerase chain reaction) amplification of soil DNA constitutes a promising domain which will in the near future contribute to the development of routine tools to monitor microbial communities in soil environments.
Tämän julkaisun valmistelusta Suomessa vastaa Suomen ympäristökeskus, puh. 040 724 8795.
The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.
The direct extraction of DNA from soil samples provides unique insight into the a- and ß-diversity of microbial communities. Next-generation sequencing of amplicons obtained by PCR (polymerase chain reaction) amplification of soil DNA constitutes a promising domain which will in the near future contribute to the development of routine tools to monitor microbial communities in soil environments.
Tämän julkaisun valmistelusta Suomessa vastaa Suomen ympäristökeskus, puh. 040 724 8795.
Sisällysluettelo
Tuoteryhmä(t)
Sidokset
Velvoittavat viittaukset
ISO/DIS 18400-206:2017 Soil quality -- Sampling -- Part 206: Collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
Tekninen komitea
Vahvistuspäivä
23.10.2020
Julkaisupäivä
27.10.2020
Painos
2
Sivumäärä
17
Julkaisun kieli
englanti