This document specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and individual chemical substances on the reproduction of the oribatid mite Oppia nitens by dermal and alimentary uptake. This chronic (28-day) test is applicable to soils and soil materials of unknown quality (e.g., contaminated sites, amended soils, soils after remediation, agricultural or other sites under concern and waste materials). This method is not intended to replace the earthworm or Collembola tests since it represents another taxonomic group (= mites; i.e., arachnids), nor the predatory mite test since this species represents a different trophic level and ecological niche. Effects of substances are assessed using standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the test soil and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) should be either an uncontaminated soil with similar properties to the soil sample to be tested (reference soil) or a standard soil (e.g., artificial soil). Information is provided on how to use this method for testing substances under temperate conditions. This document is not applicable to substances for which the air/soil partition coefficient is greater than 1, or to substances with vapour pressure exceeding 300 Pa at 25 °C. NOTE The stability of the test substance cannot be assured over the test period. No provision is made in the test method for monitoring the persistence of the substance under test.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and material
6 Apparatus
7 Procedure
7.1 Experimental design
7.2 Preparation of test mixture
7.3 Addition of the mites
7.4 Test conditions and measurements
7.5 Determination of adult survival and reproductive output
8 Calculation and expression of results
8.1 Calculation
8.2 Expression of results
9 Validity of the test
10 Statistical analysis
10.1 General
10.2 Single-concentration tests
10.3 Multi-concentration tests
11 Test report
Annex A Techniques for rearing and breeding of Oppia nitens (informative)
Annex B Determination of water holding capacity (normative)
Annex C Guidance on adjustment of pH of artificial soil (informative)
Annex D Extraction and counting of Oppia nitens (informative)
ISO 10390:2005 Soil quality -- Determination of pH
ISO 10694:1995 Soil quality -- Determination of organic and total carbon after dry combustion (elementary analysis)
ISO 11260:2018 Soil quality -- Determination of effective cation exchange capacity and base saturation level using barium chloride solution
ISO 10390:2005 Soil quality -- Determination of pH
ISO 10694:1995 Soil quality -- Determination of organic and total carbon after dry combustion (elementary analysis)
ISO 11260:2018 Soil quality -- Determination of effective cation exchange capacity and base saturation level using barium chloride solution
ISO 11265:1994 Soil quality -- Determination of the specific electrical conductivity
ISO 11277:2020 Soil quality -- Determination of particle size distribution in mineral soil material -- Method by sieving and sedimentation
ISO 11465:1993 Soil quality -- Determination of dry matter and water content on a mass basis -- Gravimetric method
ISO/DIS 18400-206:2017 Soil quality -- Sampling -- Part 206: Collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory