Microbiology of the food chain. Methods for the detection of Anisakidae L3 larvae in fish and fishery products. Part 2: Artificial digestion method (ISO 23036-2:2021)
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This document specifies a method for the detection of Anisakidae L3 larvae commonly found in marine and anadromous fishes. The method is applicable to fresh fish and/or frozen fish, as well as lightly processed fish products, such as marinated, salted or smoked. It is also suitable for visceral organs as a confirmatory method for a visual inspection scheme. The artificial digestion method[4][5][6] is applicable to quantifying parasitic infections by estimating the number of parasites in the fish musculature and, when applied to fresh fish or lightly processed fish products (never frozen before processing), determining the viability of Anisakidae L3, which can be present. This method does not apply to determining the species or genotype of detected parasites. Final identification is made by morphological and/or molecular methods.
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Sisällysluettelo
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Sample size
4.3 Sample preparation
4.4 Digestion of the sample
4.5 Filtration of the digest fluid
4.6 Verification of findings
5 Reagents
6 Equipment and consumables
7 Sampling
8 Procedure
8.1 Preparation of the sample
8.2 Preparation of the digest fluid
8.3 Digestion of the sample in the glass beaker
8.4 Filtration of the digest fluid
8.5 Microscopic examination
9 Expression of the results
10 Performance characteristics of the method
11 Test report
12 Quality assurance
Annex A Sample collection (informative)
Annex B Example of a laboratory worksheet for recording data when testing fish fillets with the artificial digestion method (informative)
882/2004 Rehu- ja elintarvikelainsäädännön sekä eläinten terveyttä ja hyvinvointia koskevien sääntöjen mukaisuuden varmistamiseksi suoritetusta virallisesta valvonnasta 2017/625